Assessment of the epidemiological status of Echinococcus multilocularis in foxes in France using ELISA coprotests on fox faeces collected in the field
Identifieur interne : 000D02 ( Main/Exploration ); précédent : 000D01; suivant : 000D03Assessment of the epidemiological status of Echinococcus multilocularis in foxes in France using ELISA coprotests on fox faeces collected in the field
Auteurs : F. Raoul [France] ; P. Deplazes [Suisse] ; N. Nonaka [Japon] ; R. Piarroux [France] ; D. A Vuitton [France] ; P. Giraudoux [France]Source :
- International Journal for Parasitology [ 0020-7519 ] ; 2001.
Descripteurs français
- Pascal (Inist)
- Wicri :
- geographic : France.
- topic : Garde à vue.
English descriptors
- KwdEn :
- Animals, Antigen, Antigens, Helminth (analysis), Coproantibody, Coproantigen, Detection, ELISA assay, Echinococciasis, Echinococcosis (epidemiology), Echinococcosis (parasitology), Echinococcosis (veterinary), Echinococcus (isolation & purification), Echinococcus multilocularis, Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (veterinary), Epidemiology, Estimation, Feces, Feces (parasitology), Field study, Fox, Foxes (parasitology), France, France (epidemiology), Host, Intestine, Small (parasitology), Parasitism, Population survey, Prevalence, Surveillance, Vulpes vulpes, Weather.
- MESH :
- chemical , analysis : Antigens, Helminth.
- geographic , epidemiology : France.
- epidemiology : Echinococcosis.
- isolation & purification : Echinococcus.
- methods : Enzyme-Linked Immunosorbent Assay.
- parasitology : Echinococcosis, Feces, Foxes, Intestine, Small.
- veterinary : Echinococcosis, Enzyme-Linked Immunosorbent Assay.
- Animals, Prevalence, Weather.
Abstract
The aim of this study was to estimate the relevance of Echinococcus multilocularis coproantigen detection in fox faeces collected in the field to identify different levels of endemicity for Echinococcus multilocularis on a large scale (n×10 km2). Six study sites were selected in a high endemicity area and two study sites in a low endemicity area in eastern France on the basis of landscape composition. Sampling was undertaken in the winters of 1996–97, 1997–98 and 1998–99. At each site, (i) necropsy and intestine examination was undertaken on a sample of shot foxes (total number of foxes, 222), and (ii) fox faeces were collected in the field along road verges, and scored for degradation status (total number of faeces, 625). Fox faeces were also sampled in a control area (n=30) in western France in the summer of 1998. Intestines were examined according to the sedimentation method. Echinococcus multilocularis coproantigens were detected by using two ELISA tests: EM-ELISA and EmA9-ELISA. The necropsy prevalence in high and low endemicity areas was 63.3% and 19.4%, respectively, and the distribution of adult worms in the fox population was highly overdispersed (75.5% of the total biomass was harboured by 11.6% of foxes). Using the two ELISA tests, there was no difference in the detection of E. multilocularis coproantigens in field faeces, regardless of the degradation status. The medians of EM- and EmA9-ELISA OD values of field faeces in high endemicity area were significantly higher than in low endemicity area (P<0.001 for both ELISA). The distribution of EM-ELISA OD values in low endemicity area was significantly higher (P=0.002) than in the control area. Moreover, for the two ELISA, the observed ELISA OD value distributions in high endemicity area, low endemicity area and control area seemed representative of the distribution of adult worms in fox populations. These results indicate that E. multilocularis coproantigen detection in field faeces could serve for large-scale surveillance, as an alternative to necropsy.
Url:
DOI: 10.1016/S0020-7519(01)00280-6
Affiliations:
- France, Japon, Suisse
- Bourgogne-Franche-Comté, Canton de Zurich, Franche-Comté
- Besançon, Zurich
- Université de Zurich
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Le document en format XML
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Six study sites were selected in a high endemicity area and two study sites in a low endemicity area in eastern France on the basis of landscape composition. Sampling was undertaken in the winters of 1996–97, 1997–98 and 1998–99. At each site, (i) necropsy and intestine examination was undertaken on a sample of shot foxes (total number of foxes, 222), and (ii) fox faeces were collected in the field along road verges, and scored for degradation status (total number of faeces, 625). Fox faeces were also sampled in a control area (n=30) in western France in the summer of 1998. Intestines were examined according to the sedimentation method. Echinococcus multilocularis coproantigens were detected by using two ELISA tests: EM-ELISA and EmA9-ELISA. The necropsy prevalence in high and low endemicity areas was 63.3% and 19.4%, respectively, and the distribution of adult worms in the fox population was highly overdispersed (75.5% of the total biomass was harboured by 11.6% of foxes). Using the two ELISA tests, there was no difference in the detection of E. multilocularis coproantigens in field faeces, regardless of the degradation status. The medians of EM- and EmA9-ELISA OD values of field faeces in high endemicity area were significantly higher than in low endemicity area (P<0.001 for both ELISA). The distribution of EM-ELISA OD values in low endemicity area was significantly higher (P=0.002) than in the control area. Moreover, for the two ELISA, the observed ELISA OD value distributions in high endemicity area, low endemicity area and control area seemed representative of the distribution of adult worms in fox populations. These results indicate that E. multilocularis coproantigen detection in field faeces could serve for large-scale surveillance, as an alternative to necropsy.</div></front></TEI><affiliations><list><country><li>France</li><li>Japon</li><li>Suisse</li></country><region><li>Bourgogne-Franche-Comté</li><li>Canton de Zurich</li><li>Franche-Comté</li></region><settlement><li>Besançon</li><li>Zurich</li></settlement><orgName><li>Université de Zurich</li></orgName></list><tree><country name="France"><noRegion><name sortKey="Raoul, F" sort="Raoul, F" uniqKey="Raoul F" first="F" last="Raoul">F. Raoul</name></noRegion><name sortKey="Giraudoux, P" sort="Giraudoux, P" uniqKey="Giraudoux P" first="P" last="Giraudoux">P. Giraudoux</name><name sortKey="Piarroux, R" sort="Piarroux, R" uniqKey="Piarroux R" first="R" last="Piarroux">R. Piarroux</name><name sortKey="Raoul, F" sort="Raoul, F" uniqKey="Raoul F" first="F" last="Raoul">F. Raoul</name><name sortKey="Vuitton, D A" sort="Vuitton, D A" uniqKey="Vuitton D" first="D. A" last="Vuitton">D. A Vuitton</name></country><country name="Suisse"><region name="Canton de Zurich"><name sortKey="Deplazes, P" sort="Deplazes, P" uniqKey="Deplazes P" first="P" last="Deplazes">P. Deplazes</name></region></country><country name="Japon"><noRegion><name sortKey="Nonaka, N" sort="Nonaka, N" uniqKey="Nonaka N" first="N" last="Nonaka">N. Nonaka</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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